ABM94548
Gab 1 (phospho Tyr627) rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Gab 1 (phospho Tyr627) rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Gab 1 (phospho Tyr627) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Gab 1.
Background
GRB2 associated binding protein 1(GAB1) Homo sapiens The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
Additional antibody information
| Specificity | Phospho-Gab 1 (Y627) Polyclonal Antibody detects endogenous levels of Gab 1 protein only when phosphorylated at Y627. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Phospho-Gab 1 (Y627) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GAB1 (Phospho-Tyr627) Antibody | Western blot analysis of GAB1 (Phospho-Tyr627) Antibody. The lane on the right is blocked with the GAB1 (Phospho-Tyr627) peptide. |
Key Facts
Product type
Primary Antibody›
Target: Gab 1
| Target | Gab 1 |
| Also known as | GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Mouse;Monkey |
| Observed band | 110kD |
| Cellular localization | cytosol, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>EGFR tyrosine kinase inhibitor resistance;>>ErbB signaling pathway;>>Ras signaling pathway;>>Phospholipase D signaling pathway;>>Neurotrophin signaling pathway;>>Bacterial invasion of epithelial cells;>>Proteoglycans in cancer;>>Renal cell carcinoma;>>Hepatocellular carcinoma;>>Gastric cancer |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr627 AA range:611-643 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Monkey |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Monkey | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
Gab 1
Also known as
GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Monkey
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GAB1 around the phosphorylation site of Tyr627 AA range:611-643
Observed band
110kD
Cellular localization
cytosol,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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