Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94035

WHRN rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

WHRN rabbit pAb from ELK Biotechnology Read more ›

WHRN rabbit pAb
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WHRN rabbit pAb

Product Description

WHRN rabbit pAb is a research-use antibody from ELK Biotechnology for studying WHRN in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is thought to function in the organization and stabilization of sterocilia elongation and actin cystoskeletal assembly, based on studies of the related mouse gene. Mutations in this gene have been associated with autosomal recessive non-syndromic deafness and Usher Syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Mar 2010],

Additional antibody information

Specificity This antibody detects endogenous levels of WHRN at Human/Mouse/Rat
Validation evidence captions Western blot analysis of lysates from CACO2 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: WHRN
Collaborator ELK Biotechnology
Partner model ES12303
Target WHRN
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human WHRN AA range: 419-469
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse
Rat

Specifications

Target
WHRN
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized peptide derived from human WHRN AA range: 419-469
Cellular localization
Cytoplasm . Cell projection, stereocilium . Cell projection, growth cone . Photoreceptor inner segment . Cell junction, synapse . Detected at the level of stereocilia in inner and outer hair cells of the cochlea and vestibule. Localizes to both tip and ankle-link stereocilia regions. Colocalizes with the growing ends of actin filaments. Colocalizes with MPP1 in the retina, at the outer limiting membrane (OLM), outer plexifirm layer (OPL), basal bodies and at the connecting cilium (CC). In photoreceptors, localizes at a plasma membrane microdomain in the apical inner segment that surrounds the connecting cilia called periciliary membrane complex. .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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