Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93996

ZBED6 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ZBED6 rabbit pAb from ELK Biotechnology Read more ›

ZBED6 rabbit pAb
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ZBED6 rabbit pAb

Product Description

ZBED6 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZBED6 in WB workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this transposon-derived intronless gene is a transcriptional repressor that binds to the consensus sequence 5'-GCTCGC-3'. The encoded protein has been shown to repress IGF2 transcription. This gene is located within the first intron of the ZC3H11A gene. [provided by RefSeq, Jul 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of ZBED6 at Human/Mouse
Validation evidence captions Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: ZBED6
Collaborator ELK Biotechnology
Partner model ES12237
Target ZBED6
Research areasAntibody Research
Reactivity Human,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from human ZBED6 AA range :130-190
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed in pancreatic islet cells (at protein level).
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000
Mouse

Specifications

Target
ZBED6
Concentration
1 mg/ml
Working concentration
WB 1:500-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse
Tested applications
WB
Immunogen
Synthesized peptide derived from human ZBED6 AA range :130-190
Cellular localization
Nucleus . Nucleus, nucleolus . Cytoplasm . Located predominantly in the nucleolus but is also dispersed throughout the nucleus (By similarity). Mainly cytoplasmic in insulin- and glucagon-positive islet pancreatic cells, however nuclear localization is evidence in some pancreatic endocrine cells (PubMed:24043816). .
Dilution range
WB 1:500-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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