Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM93825

α-E-Catenin (phospho-Ser652) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

α-E-Catenin (phospho-Ser652) rabbit pAb from ELK Biotechnology Read more ›

α-E-Catenin (phospho-Ser652) rabbit pAb
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α-E-Catenin (phospho-Ser652) rabbit pAb

Product Description

α-E-Catenin (phospho-Ser652) rabbit pAb is a research-use antibody from ELK Biotechnology for studying α-E-Catenin in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

catenin alpha 1(CTNNA1) Homo sapiens This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016],

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat α-E-Catenin (phospho-Ser652)
Validation evidence captions Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: α-E-Catenin
Collaborator ELK Biotechnology
Partner model ES12062
Target α-E-Catenin
Research areas>>Hippo signaling pathway, >>Adherens junction, >>Leukocyte transendothelial migration, >>Bacterial invasion of epithelial cells, >>Pathways in cancer, >>Endometrial cancer, >>Gastric cancer, >>Arrhythmogenic right ventricular cardiomyopathy
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human α-E-Catenin (Ser652)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Expressed ubiquitously in normal tissues.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Mouse
Rat

Specifications

Target
α-E-Catenin
Also known as
Catenin alpha-1 (Alpha E-catenin) (Cadherin-associated protein) (Renal carcinoma antigen NY-REN-13)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized phosho peptide around human α-E-Catenin (Ser652)
Observed band
100kD
Cellular localization
Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.; Isoform 3: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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