α-E-Catenin (phospho-Ser652) rabbit pAb
α-E-Catenin (phospho-Ser652) rabbit pAb from ELK Biotechnology Read more ›
Product Description
α-E-Catenin (phospho-Ser652) rabbit pAb is an ELK Biotechnology antibody for research workflows involving α-E-Catenin.
Background
catenin alpha 1(CTNNA1) Homo sapiens This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016],
Additional antibody information
| Specificity | This antibody detects endogenous levels of Human Mouse Rat α-E-Catenin (phospho-Ser652) |
|---|---|
| Validation evidence captions | Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7,using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000 |
Key Facts
| Target | α-E-Catenin |
| Also known as | Catenin alpha-1 (Alpha E-catenin) (Cadherin-associated protein) (Renal carcinoma antigen NY-REN-13) |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:1000-2000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 100kD |
| Cellular localization | Isoform 1: Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction. Found at cell-cell boundaries and probably at cell-matrix boundaries.; Isoform 3: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Purity | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>Hippo signaling pathway;>>Adherens junction;>>Leukocyte transendothelial migration;>>Bacterial invasion of epithelial cells;>>Pathways in cancer;>>Endometrial cancer;>>Gastric cancer;>>Arrhythmogenic right ventricular cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WB |
| Dilution range | WB 1:1000-2000 |
| Immunogen | Synthesized phosho peptide around human α-E-Catenin (Ser652) |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1000-2000 | — |
| Mouse | — | — |
| Rat | — | — |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.