KLOT rabbit pAb from ELK Biotechnology Read more ›
KLOT rabbit pAb is a research-use antibody from ELK Biotechnology for studying KLOT in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008],
| Specificity | KLOT Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of mouse kidney, rat kidney, HuvEc cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002 was diluted at 1:10000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES11915 |
| Target | KLOT |
| Research areas | >>Pentose and glucuronate interconversions, >>Ascorbate and aldarate metabolism, >>Metabolic pathways, >>Longevity regulating pathway, >>Parathyroid hormone synthesis, secretion and action, >>Endocrine and other factor-regulated calcium reabsorption |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000 ELISA 1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from part region of human protein AA range: 400-500 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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