ABM93734
ANF rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
ANF rabbit pAb from ELK Biotechnology Read more ›
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Product Description
ANF rabbit pAb is an ELK Biotechnology antibody for research workflows involving ANF.
Background
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015],
Additional antibody information
| Specificity | ANF Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night |
Key Facts
Product type
Primary Antibody›
Target: ANF
| Target | ANF |
| Concentration | 1 mg/ml |
| Working concentration | WB 1:500-2000 ELISA 1:5000-20000 |
| Species reactivity | Human;Mouse;Rat |
| Observed band | 16kD |
| Cellular localization | Long-acting natriuretic peptide: Secreted . Detected in blood. .; Vessel dilator: Secreted . Detected in blood. .; Kaliuretic peptide: Secreted . Detected in blood. .; Urodilatin: Secreted . Detected in urine (PubMed:2972874, PubMed:9794555, PubMed:8351194, PubMed:8779891). Not detected in blood (PubMed:8351194). Increased electrolytes, osmolality and intracellular cAMP levels increase peptide secretion/excretion (PubMed:9893117, PubMed:8853410, PubMed:8351194, PubMed:8779891). .; Atrial natriuretic peptide: Secreted . Perikaryon . Cell projection . Detected in blood (PubMed:8351194, PubMed:8779891, PubMed:7955907, PubMed:8653797, PubMed:15741263, PubMed:18835931, PubMed:2532366, PubMed:7984506). Detected in urine in one study (PubMed:8351194). However, in another study, was not |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, and 0.02% sodium azide. |
| Research area | >>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>HIF-1 signaling pathway;>>Vascular smooth muscle contraction;>>Thermogenesis;>>Oxytocin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Aldosterone synthesis and secretion;>>African trypanosomiasis |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | WB 1:500-2000 ELISA 1:5000-20000 |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
ABMIUM Validated™
ELISA
| Species | Dilution | Notes |
|---|
Specifications
Target
ANF
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
16kD
Cellular localization
Long-acting natriuretic peptide: Secreted . Detected in blood. .; Vessel dilator: Secreted . Detected in blood. .; Kaliuretic peptide: Secreted . Detected in blood. .; Urodilatin: Secreted . Detected in urine (PubMed:2972874, PubMed:9794555, PubMed:8351194, PubMed:8779891). Not detected in blood (PubMed:8351194). Increased electrolytes, osmolality and intracellular cAMP levels increase peptide secretion/excretion (PubMed:9893117, PubMed:8853410, PubMed:8351194, PubMed:8779891). .; Atrial natriuretic peptide: Secreted . Perikaryon . Cell projection . Detected in blood (PubMed:8351194, PubMed:8779891, PubMed:7955907, PubMed:8653797, PubMed:15741263, PubMed:18835931, PubMed:2532366, PubMed:7984506). Detected in urine in one study (PubMed:8351194). However, in another study, was not
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage & Stability
Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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