ANF rabbit pAb from ELK Biotechnology Read more ›
ANF rabbit pAb is a research-use antibody from ELK Biotechnology for studying ANF in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. This gene is located adjacent to another member of the natriuretic family of peptides on chromosome 1. [provided by RefSeq, Oct 2015],
| Specificity | ANF Polyclonal Antibody detects endogenous levels of protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night |
| Collaborator | ELK Biotechnology |
| Partner model | ES11830 |
| Target | ANF |
| Research areas | >>cGMP-PKG signaling pathway, >>cAMP signaling pathway, >>HIF-1 signaling pathway, >>Vascular smooth muscle contraction, >>Thermogenesis, >>Oxytocin signaling pathway, >>Regulation of lipolysis in adipocytes, >>Renin secretion, >>Aldosterone synthesis and secretion, >>African trypanosomiasis |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2000 ELISA 1:5000-20000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Urodilatin: Detected in the kidney distal tubular cells (at protein level) (PubMed:9794555, PubMed:8384600). Present in urine (at protein level) (PubMed:2972874, PubMed:9794555, PubMed:8351194, PubMed:8779891). ; Atrial natriuretic peptide: Detected in atrial and ventricular plasma samples, and in adipocytes (at protein level) (PubMed:22291141, PubMed:21672517). Detected in urine in one study (PubMed:8351194). However, was not detected in urine in another study (PubMed:7984506). In the brain, predominantly expressed in the gray matter with very weak expression in the white matter (at protein level) (PubMed:30534047). Localizes to astrocyte-like structures throughout the white matter, and in the cerebral vessels detected in the leptomeningeal and parenchymal vessels, and endothelium and |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2000 ELISA 1:5000-20000 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
