Manufactured by:ELK Biotechnology
RUO

CHIP rabbit pAb

CHIP rabbit pAb from ELK Biotechnology

SKU:
ABM103219
Manufacturer SKU
ES11301
Product type
Primary Antibodies
TargetCHIP
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse
Expression systemExpressed in differentiated myotubes (at protein level) (PubMed:17369820). Highly expressed in skeletal muscle, heart, pancreas, brain and placenta (PubMed:10330192, PubMed:11435423). Detected in kidney, liver and lung (PubMed:10330192, PubMed:11435423).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

CHIP rabbit pAb is a research-use antibody from ELK Biotechnology for studying CHIP in WB, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein containing tetratricopeptide repeat and a U-box that functions as a ubiquitin ligase/cochaperone. The encoded protein binds to and ubiquitinates shock cognate 71 kDa protein (Hspa8) and DNA polymerase beta (Polb), among other targets. Mutations in this gene cause spinocerebellar ataxia, autosomal recessive 16. Alternative splicing results in multiple transcript variants. There is a pseudogene for this gene on chromosome 2. [provided by RefSeq, Jun 2014],

Additional antibody information

Specificity CHIP Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
Ubiquitin mediated proteolysis, Protein processing in endoplasmic reticulum
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from human protein . at AA range: 160-240
Observed band
33kD
Cellular localization
Cytoplasm . Nucleus . Translocates to the nucleus in response to inflammatory signals in regulatory T-cells (Treg). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 160-240
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Manufacturer SKU
ES11301
UniProt entry
Q9UNE7
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

STUB1STIP1 homology and U-box containing protein 1

View target guide

STUB1 identifies STIP1 homology and U-box containing protein 1 in Homo sapiens (Human). This target record connects NCBI Gene 10273, UniProtKB Q9UNE7 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
10273
UniProtKB
Q9UNE7
ELK Biotechnology
£176.00