Primary Antibody ISO 9001|ABMIUM Verified™
ABM93129

SFRP1 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

SFRP1 rabbit pAb from ELK Biotechnology Read more ›

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SFRP1 rabbit pAb
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SFRP1 rabbit pAb

Product Description

SFRP1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving SFRP1.

Background

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009],

Additional antibody information

Specificity SFRP1 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product type Primary Antibody Target: SFRP1
Target SFRP1
Concentration 1 mg/ml
Working concentration WB 1:500-2000 ELISA 1:5000-20000
Species reactivity Human;Mouse;Rat
Observed band 34kD
Cellular localization Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Research area >>Wnt signaling pathway
Host species Rabbit
Clonality Polyclonal
Isotype IgG
Conjugation Unconjugated
Tested applications
WBELISA
Dilution range WB 1:500-2000 ELISA 1:5000-20000
Immunogen Synthesized peptide derived from part region of human protein
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMCHIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
SFRP1
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human;Mouse;Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
34kD
Cellular localization
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
-20°C/1 year
Storage conditions
-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ISO 9001
ISO 9001:2015 Certified

Manufactured under ISO 9001:2015 quality management standards.

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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