Cleaved-COL1A2 (G1102) rabbit pAb
Cleaved-COL1A2 (G1102) rabbit pAb from ELK Biotechnology Read more ›
Product Description
Cleaved-COL1A2 (G1102) rabbit pAb is an ELK Biotechnology antibody for research workflows involving COL1A2.
Background
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008],
Additional antibody information
| Specificity | Cleaved-COL1A2 (G1102) Polyclonal Antibody detects endogenous levels of fragment of activated COL1A2 protein resulting from cleavage adjacent to G1102. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-COL1A2 (G1102) Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of VEC cells using Cleaved-COL1A2 (G1102) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Collagen I alpha2 (Cleaved-Gly1102) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | COL1A2 |
| Also known as | COL1A2; Collagen alpha-2(I) chain; Alpha-2 type I collagen |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Species reactivity | Human;Rat;Mouse; |
| Observed band | 92kD |
| Cellular localization | Secreted, extracellular space, extracellular matrix . |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form / buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Research area | >>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Platelet activation;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Protein digestion and absorption;>>Amoebiasis;>>Human papillomavirus infection;>>Proteoglycans in cancer;>>Diabetic cardiomyopathy |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen I alpha2. AA range:1053-1102 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.