ATG5 (3C7) Mouse mAb from ELK Biotechnology Read more ›
ATG5 (3C7) Mouse mAb is a research-use antibody from ELK Biotechnology for studying ATG5 (3C7) in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200. | Western blot analysis of Hela Cell Lysate using ATG5 （EM1374）Mouse mAb diluted at 1:10000. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1374 |
| Target | ATG5 (3C7) |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000-10,000 IHC 1:100-200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant Protein |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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