ATG5 (3C7) Mouse mAb
ATG5 (3C7) Mouse mAb from ELK Biotechnology Read more ›
Product Description
ATG5 (3C7) Mouse mAb is an ELK Biotechnology antibody for research workflows involving ATG5 (3C7).
Background
Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Additional antibody information
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 （EM1374）Mouse mAb diluted at 1:200. | Western blot analysis of Hela Cell Lysate using ATG5 （EM1374）Mouse mAb diluted at 1:10000. |
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Key Facts
| Target | ATG5 (3C7) |
| Also known as | APG5, APG5L, ASP, Autophagy protein 5 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000-10,000 IHC 1:100-200 |
| Species reactivity | Human, Mouse, Rat |
| Observed band | 55kDa |
| Cellular localization | Cytoplasm, Membrane |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:5,000-10,000 IHC 1:100-200 |
| Immunogen | Recombinant Protein |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.