Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92460

β-Tubulin Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

β-Tubulin Mouse mAb from ELK Biotechnology Read more ›

β-Tubulin Mouse mAb
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β-Tubulin Mouse mAb

Product Description

β-Tubulin Mouse mAb is a research-use antibody from ELK Biotechnology for studying β-Tubulin in WB, IHC, IF workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Additional antibody information

Validation evidence captions Western blot analysis of Zebrafish skeletal muscle with β-tubulin Mouse mAb(Zebrafish Specific) diluted at 1:5,000.

Key Facts

Product typePrimary Antibody Target: β-Tubulin
Collaborator ELK Biotechnology
Partner model EM1114
Target β-Tubulin
Research areasAntibody Research
Reactivity Zebrafish
Applications WB,IHC,IF
ConjugationUnconjugated
Dilution rangeWB 1:5,000-20,000 IHC 1:200 IF 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Zebrafish
ABMIUM Validated™ WB
Species Dilution Notes
Zebrafish WB 1:5
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
β-Tubulin
Concentration
1mg/mL
Working concentration
WB 1:5,000-20,000 IHC 1:200 IF 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Zebrafish
Tested applications
WBIHCIF
Immunogen
Synthetic Peptide
Observed band
55kDa
Dilution range
WB 1:5,000-20,000 IHC 1:200 IF 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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