Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92422

Ki 67 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

Ki 67 Mouse mAb from ELK Biotechnology Read more ›

Ki 67 Mouse mAb
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Ki 67 Mouse mAb

Product Description

Ki 67 Mouse mAb is a research-use antibody from ELK Biotechnology for studying Ki 67 in IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells (G0).

Additional antibody information

Validation evidence captions IHC staining of Mouse Kidney tissue with Ki 67 mouse mAb(4A8) diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: Ki 67
Collaborator ELK Biotechnology
Partner model EM1064
Target Ki 67
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications IHC
ConjugationUnconjugated
Dilution rangeIHC 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
Human IHC 1:200
Rat
Mouse

Specifications

Target
Ki 67
Also known as
KIA, MKI67
Concentration
1mg/mL
Working concentration
IHC 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
IHC
Immunogen
Synthetic Peptide
Cellular localization
Chromosome, Nucleus
Dilution range
IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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