Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92413

CK8 Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

CK8 Mouse mAb from ELK Biotechnology Read more ›

CK8 Mouse mAb
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CK8 Mouse mAb

Product Description

CK8 Mouse mAb is a research-use antibody from ELK Biotechnology for studying CK8 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)Mouse Brain with CK8 mAb diluted at 1:4,000. | IHC staining of human colon cancer tissue with CK8 mouse mAb(8G8) diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: CK8
Collaborator ELK Biotechnology
Partner model EM1055
Target CK8
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:2,000-5,000 IHC 1:200
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:2
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
CK8
Also known as
CARD2, CYK8, Cytokeratin 8, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8
Concentration
1mg/mL
Working concentration
WB 1:2,000-5,000 IHC 1:200
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
54kDa
Cellular localization
Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Dilution range
WB 1:2,000-5,000 IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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