CK7 Mouse mAb
CK7 Mouse mAb from ELK Biotechnology Read more ›
Product Description
CK7 Mouse mAb is an ELK Biotechnology antibody for research workflows involving CK7.
Background
Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2)Mouse Kidney, 3) Mouse Brain with CK7 mAb diluted at 1:2,000. | IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1054 1:1,000 Secondary antibody: Goat anti-Mouse IgG Light chain specific(S003), 1:5,000 |
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Key Facts
| Target | CK7 |
| Also known as | Cytokeratin 7, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7 |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200 IP 1:200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 54kDa |
| Cellular localization | Cytoplasm |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIP |
| Dilution range | WB 1:1,000-2,000 IHC 1:200 IP 1:200 |
| Immunogen | Synthetic Peptide |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.