CK7 Mouse mAb from ELK Biotechnology Read more ›
CK7 Mouse mAb is a research-use antibody from ELK Biotechnology for studying CK7 in WB, IHC, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.
| Validation evidence captions | Western blot analysis of 1) Hela, 2)Mouse Kidney, 3) Mouse Brain with CK7 mAb diluted at 1:2,000. | IHC staining of human lung cancer tissue with CK7 mouse mAb(12D7) diluted at 1:200. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1054 1:1,000 Secondary antibody: Goat anti-Mouse IgG Light chain specific(S003), 1:5,000 |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1054 |
| Target | CK7 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-2,000 IHC 1:200 IP 1:200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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