GAPDH Mouse mAb from ELK Biotechnology Read more ›
GAPDH Mouse mAb is a research-use antibody from ELK Biotechnology for studying GAPDH in WB, IHC, IF workflows with listed reactivity for Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast. Specifications and supporting documents below should be reviewed when planning the experiment.
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
| Validation evidence captions | Western blot analysis of Hela (1), Rat brain (2), Rabbit Muscle(3), Sheep Muscle(4), and Mouse brain (5) with GAPDH mouse mAb(2B8) diluted at 1:10,000. | Western blot analysis of Chicken Muscle (1), Hamster Lung (2), Pig Heart(3), with GAPDH mouse mAb(2B8) diluted at 1:10,000. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1028 |
| Target | GAPDH |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse,Monkey,Dog,Chicken,Hamster,Rabbit,Pig,Sheep,Insect,Yeast |
| Applications | WB,IHC,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 IHC 1:200 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse | |||||||
| Monkey | |||||||
| Dog | |||||||
| Chicken | |||||||
| Hamster | |||||||
| Rabbit | |||||||
| Pig | |||||||
| Sheep | |||||||
| Insect | |||||||
| Yeast |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Rat | — | — |
| Mouse | — | — |
| Monkey | — | — |
| Dog | — | — |
| Chicken | — | — |
| Hamster | — | — |
| Rabbit | — | — |
| Pig | — | — |
| Sheep | — | — |
| Insect | — | — |
| Yeast | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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