C-Myc-Tag Mouse mAb from ELK Biotechnology Read more ›
C-Myc-Tag Mouse mAb is a research-use antibody from ELK Biotechnology for studying C-Myc-Tag in WB, IF, IP workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a transcription factor that activates expression of a great number of genes through binding on consensus sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent study demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a potential cancer drug target.
| Validation evidence captions | IP antibody use：3ug Flag Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with C-Myc-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- C-Myc mAb+ P | 1ug C-Myc fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 | IF analysis of 293 cells transfected with a C-Myc-tag protein, using ELKbio anti-C-MycTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-C-Myc) |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1001 |
| Target | C-Myc-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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