Flag-Tag Mouse mAb from ELK Biotechnology Read more ›
Flag-Tag Mouse mAb is a research-use antibody from ELK Biotechnology for studying Flag-Tag in WB, IF, IP workflows with listed reactivity for Epitope tag. Specifications and supporting documents below should be reviewed when planning the experiment.
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
| Validation evidence captions | IP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with Flag-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- Flag mAb+ P | 1ug Flag fusion protein+ Primary antibody dilution at 1:10,000. | IF analysis of 293 cells transfected with a Flag-tag protein, using ELKbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag) |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1000 |
| Target | Flag-Tag |
| Research areas | Antibody Research |
| Reactivity | Epitope tag |
| Applications | WB,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:5,000 IP 1:200 IF 1:2,000 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Epitope tag |
| Species | Dilution | Notes |
|---|---|---|
| Epitope tag | WB 1:5 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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