Rat MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

Rat MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of MAP1LC3B.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include LC3B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP.