Human ARNT(Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit from ELK Biotechnology
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Human ARNT(Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ARNT in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARNT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARNT in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include HIF-1beta; bHLHe2; Hypoxia Inducible Factor 1 Beta; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; Hypoxia-inducible factor 1-beta.
Fully tested in-house by ABMIUM, or supported by an advanced validation report from a partner reviewed by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Tissue homogenates | Cell lysates | Other biological fluids |
|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Aryl hydrocarbon receptor nuclear translocator (ARNT; HIF-1β/HIF1B) is a bHLH-PAS transcriptional partner that links AHR xenobiotic signalling with HIF hypoxia responses. This catalogue-backed ABMIUM record links human ARNT (NCBI Gene 405; UniProt P27540) to research reagents.
Biological function: ARNT forms obligate heterodimers with other bHLH-PAS transcription factors. With ligand-activated AHR it supports xenobiotic-response-element transcription, while heterodimerisation with HIF alpha subunits enables hypoxia-response-element transcription controlling adaptive programmes such as angiogenesis, erythropoiesis and metabolic responses.
