Rat FCN1(Ficolin 1) Microsample ELISA Kit
Rat FCN1(Ficolin 1) Microsample ELISA Kit from ELK Biotechnology Read more ›
Product Description
Rat FCN1(Ficolin 1) Microsample ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of FCN1.
Test principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FCN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Target background
Alternative names include FCNM; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-A; M-ficolin.
Key Facts
| Target | FCN1 |
| Also known as | FCNM; Collagen/fibrinogen domain-containing protein 1; Ficolin-alpha; Ficolin-A; M-ficolin |
| Species reactivity | Rat |
| Form / buffer | Pre-coated microplate kit |
| Research area | Metabolic pathway, Infection immunity, Bone metabolism |
| Assay type | Sandwich |
| Detection method | Colorimetric (TMB/HRP) |
| Sensitivity | 1.9 ng/mL |
| Detection range | 4.69-300 ng/mL |
| Sample types |
SerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
See sample validation ›
|
| Plate format | 96-well pre-coated |
Sample Type Validation
1 sample type| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT | |
|---|---|---|---|---|---|---|
| Sample compatibility |
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.