Manufactured by:ELK Biotechnology
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Human VTCN1(V-Set Domain Containing T-Cell Activation Inhibitor 1) ELISA Kit

Human VTCN1(V-Set Domain Containing T-Cell Activation Inhibitor 1) ELISA Kit from ELK Biotechnology

SKU:
ABM63689
Manufacturer SKU
ELK3521
Product type
ELISA Kits
TargetVTCN1
ApplicationsELISA
Species / reactivityHuman
Sample typeTissue homogenates, Cell lysates, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human VTCN1(V-Set Domain Containing T-Cell Activation Inhibitor 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring VTCN1 in Tissue homogenates, Cell lysates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VTCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VTCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VTCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VTCN1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include B7-H4; B7H4; B7S1; B7X; B7h.5; VCTN1; T-cell costimulatory molecule B7x; Immune costimulatory protein B7-H4.

Key facts & specifications
Research areas
Signal Transduction
Also known as
B7-H4; B7H4; B7S1; B7X; B7h.5; VCTN1; T-cell costimulatory molecule B7x; Immune costimulatory protein B7-H4
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.057 ng/mL
Detection range
0.16-10 ng/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeTissue homogenatesCell lysatesOther biological fluids
Sample compatibility
Identifiers & database references
Manufacturer SKU
ELK3521
UniProt entry
Q7Z7D3
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

VTCN1V-set domain containing T cell activation inhibitor 1

View target guide

VTCN1 identifies V-set domain containing T cell activation inhibitor 1 in Homo sapiens (Human). This target record connects NCBI Gene 79679, UniProtKB Q7Z7D3 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
79679
UniProtKB
Q7Z7D3
ELK Biotechnology
£284.00£227.20