Mouse F12(Coagulation Factor XII) Microsample ELISA Kit from ELK Biotechnology Read more ›
Mouse F12(Coagulation Factor XII) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring F12 in Plasma samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F12 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include FXII; HAF; Contact Factor; Hageman Factor.
| Collaborator | ELK Biotechnology |
| Partner model | ELK3206MS |
| Target | F12 |
| Research areas | Metabolic pathway, Hematology |
| Reactivity | Mouse |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Plasma |
| Detection range | 62.5-4000 pg/mL |
| Sensitivity | 26 pg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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