ABM92246
P21 Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
P21 Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
P21 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving P21.
Background
p21 / WAF1 / CIP1 also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1 is a protein that in humans is encoded by the CDKN1A gene. p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) 293T, 2)MCF7, 3)Mouse Liver Tissue, 4) Rat Liver Tissue with P21 Rabbit pAb EA236 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using P21 (EA236)Rabbit pAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: P21
| Target | P21 |
| Also known as | CDKN1 antibody, CDKI antibody, CDKN1A antibody, MDA 6 antibody, p21 cip1 antibody, p21waf antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 21kDa |
| Cellular localization | Cytoplasm, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
P21
Also known as
CDKN1 antibody, CDKI antibody, CDKN1A antibody, MDA 6 antibody, p21 cip1 antibody, p21waf antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
21kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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