Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92246

P21 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

P21 Rabbit pAb from ELK Biotechnology Read more ›

P21 Rabbit pAb
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P21 Rabbit pAb

Product Description

P21 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying P21 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

p21 / WAF1 / CIP1 also known as cyclin-dependent kinase inhibitor 1 or CDK-interacting protein 1 is a protein that in humans is encoded by the CDKN1A gene. p21 is a potent cyclin-dependent kinase inhibitor (CKI). The p21 (CIP1/WAF1) protein binds to and inhibits the activity of cyclin-CDK2, -CDK1, and -CDK4/6 complexes, and thus functions as a regulator of cell cycle progression at G1 and S phase.

Additional antibody information

Validation evidence captions Western blot analysis of 1) 293T, 2)MCF7, 3)Mouse Liver Tissue, 4) Rat Liver Tissue with P21 Rabbit pAb EA236 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using P21 (EA236)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: P21
Collaborator ELK Biotechnology
Partner model EA236
Target P21
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
P21
Also known as
CDKN1 antibody, CDKI antibody, CDKN1A antibody, MDA 6 antibody, p21 cip1 antibody, p21waf antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
21kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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