Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92233

Caspase 3 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Caspase 3 Rabbit pAb from ELK Biotechnology Read more ›

Caspase 3 Rabbit pAb
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Caspase 3 Rabbit pAb

Product Description

Caspase 3 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Caspase 3 in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP).

Additional antibody information

Validation evidence captions Western blot analysis of Hela with Caspase 3 Rabbit pAb EA221 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Tonsil Tissue using Caspase 3 (EA221)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Caspase 3
Collaborator ELK Biotechnology
Partner model EA221
Target Caspase 3
Research areasAntibody Research
Reactivity Human
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Caspase 3
Also known as
APOPAIN antibody, CASP 3 antibody, CPP32 antibody, Cysterine protease CPP32 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
32kDa
Cellular localization
Cytoplasm
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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