ABM92228
c-Raf Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
c-Raf Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
c-Raf Rabbit pAb is an ELK Biotechnology antibody for research workflows involving c-Raf.
Background
A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases.
Additional antibody information
| Validation evidence captions | Western blot analysis of Hela with c-Raf Rabbit pAb EA215 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using c-Raf （EA215）Rabbit pAb diluted at 1:200. |
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Key Facts
Product type
Primary Antibody›
Target: c-Raf
| Target | c-Raf |
| Also known as | c-Raf proto-oncogene, serine/threonine kinase antibody, EC2.7.11.1 antibody, NS5 antibody, Raf 1 antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:100-200 |
| Species reactivity | Human |
| Observed band | 75kDa |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:100-200 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
1 species| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
c-Raf
Also known as
c-Raf proto-oncogene, serine/threonine kinase antibody, EC2.7.11.1 antibody, NS5 antibody, Raf 1 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
75kDa
Dilution range
WB 1:1,000-2,000 IHC 1:100-200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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