Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92228

c-Raf Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

c-Raf Rabbit pAb from ELK Biotechnology Read more ›

c-Raf Rabbit pAb
Zoom
c-Raf Rabbit pAb

Product Description

c-Raf Rabbit pAb is a research-use antibody from ELK Biotechnology for studying c-Raf in WB, IHC workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases.

Additional antibody information

Validation evidence captions Western blot analysis of Hela with c-Raf Rabbit pAb EA215 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using c-Raf (EA215)Rabbit pAb diluted at 1:200.

Key Facts

Product typePrimary Antibody Target: c-Raf
Collaborator ELK Biotechnology
Partner model EA215
Target c-Raf
Research areasAntibody Research
Reactivity Human
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
c-Raf
Also known as
c-Raf proto-oncogene, serine/threonine kinase antibody, EC2.7.11.1 antibody, NS5 antibody, Raf 1 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
75kDa
Dilution range
WB 1:1,000-2,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.