Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92218

PI3 Kinase P85α Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PI3 Kinase P85α Rabbit pAb from ELK Biotechnology Read more ›

PI3 Kinase P85α Rabbit pAb
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PI3 Kinase P85α Rabbit pAb

Product Description

PI3 Kinase P85α Rabbit pAb is a research-use antibody from ELK Biotechnology for studying PI3 Kinase P85α in WB workflows with listed reactivity for Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Phosphatidylinositol-4,5-bisphosphate 3-kinase (also called phosphatidylinositide 3-kinases, phosphatidylinositol-3-kinases, PI 3-kinases, PI(3)Ks, PI-3Ks or by the HUGO official stem symbol for the gene family, PI3K(s)) are a family of enzymes involved in cellular functions such as cell growth, proliferation, differentiation, motility, survival and intracellular trafficking, which in turn are involved in cancer.

Additional antibody information

Validation evidence captions Western blot analysis of 1) 3T3, 2) Raw264.7, 3)Rat Kidney Tissue, 4) Rat Liver Tissue, with PI3 Kinase p85α Rabbit pAb EA199 diluted at 1:2,000. | Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2),RAW267 (Lane 3), Rat Kidney (Lane 4), using PI3 Kinase p85α diluted at 1:8,000.

Key Facts

Product typePrimary Antibody Target: PI3 Kinase P85α
Collaborator ELK Biotechnology
Partner model EA199
Target PI3 Kinase P85α
Research areasAntibody Research
Reactivity Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Rat WB 1:1
Mouse

Specifications

Target
PI3 Kinase P85α
Also known as
GRB1 antibody, p85 alpha antibody, PI3K antibody, PIK3R1 antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Rat,Mouse
Tested applications
WB
Immunogen
Recombinant Protein
Observed band
85kDa
Cellular localization
Cytosol,Endoplasmic reticulum,Golgi apparatus,Nucleus,Plasma Membrane
Dilution range
WB 1:1,000-2,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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