ABM92171
Histone H2A.X(Acetyl Lys5) Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
Histone H2A.X(Acetyl Lys5) Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
Histone H2A.X(Acetyl Lys5) Rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H2A.X(Acetyl Lys5).
Background
Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
Additional antibody information
| Validation evidence captions | Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1µM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit pAb (upper) EA116 1:2,000 and β-actin Mouse mAb (lower) EM1031 1:5,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H2A.X (Acetyl Lys5)（E116）Rabbit pAb diluted at 1:500. |
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Key Facts
Product type
Primary Antibody›
Target: Histone H2A.X(Acetyl Lys5)
| Target | Histone H2A.X(Acetyl Lys5) |
| Also known as | H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody |
| Concentration | 1mg/mL |
| Working concentration | WB 1:1,000-2,000 IHC 1:200-500 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 15kDa |
| Cellular localization | Nucleus,Chromosome |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Immunogen | Synthetic Peptide |
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Citations
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
Specifications
Target
Histone H2A.X(Acetyl Lys5)
Also known as
H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
Antiserum
Conjugation
Unconjugated
Species reactivity
Human, Rat, Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
15kDa
Cellular localization
Nucleus,Chromosome
Dilution range
WB 1:1,000-2,000 IHC 1:200-500
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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