Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92164

β Tubulin Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

β Tubulin Rabbit pAb from ELK Biotechnology Read more ›

β Tubulin Rabbit pAb
Zoom
β Tubulin Rabbit pAb

Product Description

β Tubulin Rabbit pAb is a research-use antibody from ELK Biotechnology for studying β Tubulin in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)Mouse Brain, 3) Rat Brain tissue with EA058 diluted at 1:5,000. | Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using β-tubulin(EA058) Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: β Tubulin
Collaborator ELK Biotechnology
Partner model EA058
Target β Tubulin
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:2,000-5,000 IHC 1:500-1,000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
Why ABMIUM?
2,000+ Researchers trust us
50,000+ Citations
ISO 9001 Certified Collaborators
Independently handpicked Products selected for quality and fit

Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:2
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
β Tubulin
Also known as
Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain
Concentration
1mg/mL
Working concentration
WB 1:2,000-5,000 IHC 1:500-1,000
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
55kDa
Cellular localization
Cytoplasm,Cytoskeleton,Microtubule
Dilution range
WB 1:2,000-5,000 IHC 1:500-1,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

Customer Reviews

No customer reviews yet.