β Tubulin Rabbit pAb
β Tubulin Rabbit pAb from ELK Biotechnology Read more ›
Product Description
β Tubulin Rabbit pAb is an ELK Biotechnology antibody for research workflows involving β Tubulin.
Background
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.
Additional antibody information
| Validation evidence captions | Western blot analysis of 1) Hela, 2)Mouse Brain, 3) Rat Brain tissue with EA058 diluted at 1:5,000. | Immunohistochemical analysis of paraffin-embedded Mouse Heart Tissue using β-tubulin(EA058) Rabbit pAb diluted at 1:500. |
|---|
Key Facts
| Target | β Tubulin |
| Also known as | Beta 4 tubulin, TBB5, TUBB2, TUBB2A, tubulin beta 2A, beta tubulin, tubulin beta chain |
| Concentration | 1mg/mL |
| Working concentration | WB 1:2,000-5,000 IHC 1:500-1,000 |
| Species reactivity | Human, Rat, Mouse |
| Observed band | 55kDa |
| Cellular localization | Cytoplasm,Cytoskeleton,Microtubule |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHC |
| Dilution range | WB 1:2,000-5,000 IHC 1:500-1,000 |
| Immunogen | Recombinant Protein |
Reactivity & Application Validation
3 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:2 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Specifications
Storage & Stability
Compliance & Certifications
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.