Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92150

Lamin B1 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Lamin B1 Rabbit pAb from ELK Biotechnology Read more ›

Lamin B1 Rabbit pAb
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Lamin B1 Rabbit pAb

Product Description

Lamin B1 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Lamin B1 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Additional antibody information

Validation evidence captions Western blot analysis of 1) 293T, 2)Mouse Brain, 3) Rat Brain tissue with EA041 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using Lamin B1 (EA041) Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Lamin B1
Collaborator ELK Biotechnology
Partner model EA041
Target Lamin B1
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-3,000 IHC: 1:500-1,000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Lamin B1
Also known as
ADLD, LMB1,LMNB1, MGC111419, OTTHUMP00000159218
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IHC: 1:500-1,000
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
68,45kDa
Cellular localization
Mitochondrion inner membrane
Dilution range
WB 1:1,000-3,000 IHC: 1:500-1,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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