Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92136

GAPDH Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GAPDH Rabbit pAb from ELK Biotechnology Read more ›

GAPDH Rabbit pAb
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GAPDH Rabbit pAb

Product Description

GAPDH Rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAPDH in WB, IHC workflows with listed reactivity for Zebrafish. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Additional antibody information

Validation evidence captions Western blot analysis of Zebrafish skeletal muscle with GAPDH Rabbit pAb(Zebrafish Specific) diluted at 1:5,000.

Key Facts

Product typePrimary Antibody Target: GAPDH
Collaborator ELK Biotechnology
Partner model EA027
Target GAPDH
Research areasAntibody Research
Reactivity Zebrafish
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:5,000-10,000 IHC 1:200 IF 1:200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Zebrafish
ABMIUM Validated™ WB
Species Dilution Notes
Zebrafish WB 1:5
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
GAPDH
Also known as
G3PD, G3PDH, GAPD, Glyceraldehyde 3 phosphoate dehycdrogenase, MGC102544
Concentration
1mg/mL
Working concentration
WB 1:5,000-10,000 IHC 1:200 IF 1:200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Zebrafish
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
37kDa
Dilution range
WB 1:5,000-10,000 IHC 1:200 IF 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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