ABM92124
GAPDH Rabbit pAb
ISO 9001|ABMIUM Verified™|Antibody
GAPDH Rabbit pAb from ELK Biotechnology Read more ›
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Product Description
GAPDH Rabbit pAb is an ELK Biotechnology antibody for research workflows involving GAPDH.
Background
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Additional antibody information
| Validation evidence captions | Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5) with GAPDH rabbit polyclonal diluted at 1:20,000. |
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Key Facts
Product type
Primary Antibody›
Target: GAPDH
| Target | GAPDH |
| Concentration | 1mg/mL |
| Working concentration | WB 1:5,000 IHC 1:200 |
| Species reactivity | Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus |
| Observed band | 37kDa |
| Cellular localization | Cytoplasm, Cytoskeleton, Membrane, Nucleus |
| Form / buffer | Antibody solution |
| Research area | Antibody Research |
| Host species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Tested applications |
WBIHCIF |
| Dilution range | WB 1:5,000 IHC 1:200 |
| Immunogen | Recombinant Protein |
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Citations
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Reactivity & Application Validation
8 speciess| Species | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat | |||||||
| Rabbit | |||||||
| Chicken | |||||||
| Monkey | |||||||
| Sheep | |||||||
| Xenopus |
ABMIUM Validated™
WB
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:5 | — |
| Mouse | — | — |
| Rat | — | — |
| Rabbit | — | — |
| Chicken | — | — |
| Monkey | — | — |
| Sheep | — | — |
| Xenopus | — | — |
ABMIUM Validated™
IHC
| Species | Dilution | Notes |
|---|
ABMIUM Validated™
IF
| Species | Dilution | Notes |
|---|
Specifications
Target
GAPDH
Concentration
1mg/mL
Working concentration
WB 1:5,000 IHC 1:200
Form / buffer
Antibody solution
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus
Tested applications
WBIHCIF
Immunogen
Recombinant Protein
Observed band
37kDa
Cellular localization
Cytoplasm, Cytoskeleton, Membrane, Nucleus
Dilution range
WB 1:5,000 IHC 1:200
Storage & Stability
Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
ISO 9001
ISO 9001:2015 Certified
Manufactured under ISO 9001:2015 quality management standards.
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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