Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92124

GAPDH Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GAPDH Rabbit pAb from ELK Biotechnology Read more ›

GAPDH Rabbit pAb
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GAPDH Rabbit pAb

Product Description

GAPDH Rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAPDH in WB, IHC, IF workflows with listed reactivity for Human, Mouse, Rat, Rabbit, Chicken, Monkey, Sheep, Xenopus. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Additional antibody information

Validation evidence captions Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5) with GAPDH rabbit polyclonal diluted at 1:20,000.

Key Facts

Product typePrimary Antibody Target: GAPDH
Collaborator ELK Biotechnology
Partner model EA015
Target GAPDH
Research areasAntibody Research
Reactivity Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,Xenopus
Applications WB,IHC,IF
ConjugationUnconjugated
Dilution rangeWB 1:5,000 IHC 1:200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

8 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Rabbit
Chicken
Monkey
Sheep
Xenopus
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:5
Mouse
Rat
Rabbit
Chicken
Monkey
Sheep
Xenopus
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes

Specifications

Target
GAPDH
Concentration
1mg/mL
Working concentration
WB 1:5,000 IHC 1:200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat,Rabbit,Chicken,Monkey,Sheep,Xenopus
Tested applications
WBIHCIF
Immunogen
Recombinant Protein
Observed band
37kDa
Cellular localization
Cytoplasm, Cytoskeleton, Membrane, Nucleus
Dilution range
WB 1:5,000 IHC 1:200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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