Assay Reagent supplied by Boster Bio Read more ›
Citrate Buffer Powder is a assay reagent from Boster Bio intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.
Boster’s Citrate Buffer Pack is an IHC antigen retrieval reagent used in heat induced epitope retrieval (HIER) for recovery of antigens masked by cross-linking during fixation.In immunohistochemistry (IHC) the most commonly used fixatives for preserving cell morphology and tissue architecture cause cross-linkages that mask epitopes, leading to poor or no staining. To reverse the loss of antigenicity and enable the primary antibodies to bind antigens, Heat-Induced Epitope Retrieval (HIER) is used to unmask epitopes by breaking the methylene bridges formed during fixation. During the process of HIER the buffer helps unfold the proteins, improving the accessibility of antibodies to tissue antigens. Citrate buffer solution is the preferred antigen retrieval buffer for most antibodies during HIER.
| Collaborator | Boster Bio |
| Partner model | AR0024 |
| Applications | IHC |
| Shelf life | Store at room temperature (RT) for one year |
| Availability | Available |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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