ABM-27083
Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72)
ABMIUM Verified™|Collaborator Tested|Boster Bio
Primary Antibody supplied by Boster Bio Read more ›
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Product Description
Boster Bio Anti-p27Kip1 CDKN1B Antibody (Monoclonal, DCS-72) catalog # MA1076. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
P27 (kip1), also known as cyclin dependent kinase inhibitor 1B (CDKN1B), is a major target of AFX-like forkhead proteins. CDKN1B (p27) belongs to the Cip/Kip family and functions as an important cell cycle gatekeeper. Phosphorylation leads to the ubiquitination and degradation of CDKN1B. P27 (kip1) mapped to 12p13. An increase in p27 causes proliferating cells to exit from the cell cycle, and a decrease in p27 is necessary for quiescent cells to resume division. Abnormally low amounts of p27 are associated with pathological states of excessive cell proliferation, especially cancers. Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.
Product information
| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | MA1076 |
| Product category | Primary Antibodies, Mouse Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | IF/ICC, WB |
| Available conjugations | Unconjugated |
| Gene name | CDKN1B |
| Protein name | Cyclin-dependent kinase inhibitor 1B |
| Specificity | No cross reactivity with other proteins. |
| Immunogen | recombinant rodent p27Kip1 protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Purification | Ascites |
| Storage | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| UniProt | O08769 |
| NCBI Gene ID | Human(1027), Mouse(12576), Rat(83571) |
Available variants
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μg/vial |
Key Facts
Product type
Primary Antibody›
Target: Cyclin-dependent kinase inhibitor 1B
| Target | Cyclin-dependent kinase inhibitor 1B |
| Also known as | KIP1; MEN4; CDKN4; MEN1B; P27KIP1; Cyclin-dependent kinase inhibitor p27 |
| Concentration | Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
| Species reactivity | Human,Mouse,Rat |
| Observed band | 18 kDa |
| Purity | No cross reactivity with other proteins. |
| Form / buffer | Lyophilized |
| Shelf life | Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles. |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone number | Clone: DCS-72 |
| Isotype | Mouse IgG1 |
| Conjugation | Unconjugated |
| Non-cross-reactive with | No cross-reactivity with other proteins |
| Tested applications |
IF/ICCWB |
| Dilution range | Immunocytochemistry , 1μg/ml, Human, mouse, rat, - Western blot, 2-4μg/ml, Human, mouse, rat |
| Immunogen | recombinant rodent p27Kip1 protein. |
| Purification | Ascites |
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Reactivity & Application Validation
3 speciess| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | FLOW CYTOMETRY | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Specifications
Target
Cyclin-dependent kinase inhibitor 1B
Also known as
KIP1; MEN4; CDKN4; MEN1B; P27KIP1; Cyclin-dependent kinase inhibitor p27
Concentration
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Mouse
Clonality
Monoclonal
Clone number
Clone: DCS-72
Isotype
Mouse IgG1
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
IF/ICCWB
Immunogen
recombinant rodent p27Kip1 protein.
Observed band
18 kDa
Dilution range
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 2-4μg/ml, Human, mouse, rat
Western blot, 2-4μg/ml, Human, mouse, rat
Purification
Ascites
Storage & Stability
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3. *This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Compliance & Certifications
RUO
For Research Use Only
Not intended for diagnostic or therapeutic use.
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