Primary Antibody ABMIUM Verified™Collaborator Tested
ABM-22712

Anti-EIF4G1 Antibody Picoband®

ABMIUM Verified™|Collaborator Tested|Boster Bio

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Anti-EIF4G1 Antibody Picoband® Boster Bio
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Anti-EIF4G1 Antibody Picoband® Boster Bio

Product Description

Boster Bio Anti-EIF4G1 Antibody Picoband® catalog # A01351-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Eukaryotic translation initiation factor 4 gamma 1 is a protein that in humans is encoded by the EIF4G1 gene. It is mapped to 3q27.1. The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly (A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference A01351-2
Product category Primary Antibodies
Reactive species Human, Mouse, Rat
Tested applications ELISA, Flow Cytometry, IF/ICC, IHC, IHC-P, WB
Available conjugations Unconjugated, Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, Fluoro647
Gene name EIF4G1
Protein name Eukaryotic translation initiation factor 4 gamma 1
Specificity No cross reactivity with other proteins.
Immunogen E.coli-derived human EIF4G1 recombinant protein (Position: Q497-R681+E1409-L1515).
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Purification Immunogen affinity purified.
Storage Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
UniProt Q04637
NCBI Gene ID 1981

Available variants

Conjugation Size
Unconjugated 100 μg/vial
Biotin 100 μg/vial
Cy3 100 μg/vial
Fluoro488 100 μg/vial
Fluoro550 100 μg/vial
Fluoro594 100 μg/vial
FITC 100 μg/vial
HRP 100 μg/vial
APC 100 μg/vial
PE 100 μg/vial
Fluoro647 100 μg/vial

Key Facts

Product type Primary Antibody Target: Eukaryotic translation initiation factor 4 gamma 1
Target Eukaryotic translation initiation factor 4 gamma 1
Also known as eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4F; EIF4G; EIF4G1; EIF4GI; p220
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Species reactivity Human,Mouse,Rat
Observed band 250 kDa
Purity No cross reactivity with other proteins.
Form / buffer Lyophilized
Shelf life Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Host species Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Conjugation Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Non-cross-reactive with No cross-reactivity with other proteins.
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Dilution range Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Immunogen E.coli-derived human EIF4G1 recombinant protein (Position: Q497-R681+E1409-L1515).
Purification Immunogen affinity purified.
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Reactivity & Application Validation

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFLOW CYTOMETRYELISAIP
Human
Mouse
Rat

Specifications

Target
Eukaryotic translation initiation factor 4 gamma 1
Also known as
eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4F; EIF4G; EIF4G1; EIF4GI; p220
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Purity
No cross reactivity with other proteins.
Form / buffer
Lyophilized
Host species
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Conjugation
Unconjugated|Biotin|Cy3|Fluoro488|Fluoro550|Fluoro594|FITC|HRP|APC|PE|Fluoro647
Species reactivity
Human,Mouse,Rat
Tested applications
ELISAFlow CytometryIF/ICCIHCIHC-PWB
Immunogen
E.coli-derived human EIF4G1 recombinant protein (Position: Q497-R681+E1409-L1515).
Observed band
250 kDa
Dilution range
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Purification
Immunogen affinity purified.

Storage & Stability

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Long-term storage
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage conditions
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
Storage buffer
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Shelf life
Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

RUO
For Research Use Only

Not intended for diagnostic or therapeutic use.

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