Primary Antibody supplied by Boster Bio Read more ›
Anti-EIF4E Monoclonal Antibody is a research-use antibody from Boster Bio for studying Eukaryotic translation initiation factor 4E in Flow Cytometry, IHC, IP, WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Boster Bio Anti-EIF4E Monoclonal Antibody catalog # M00135. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.| Supplier | Boster Bio |
|---|---|
| ABMIUM evidence status | ABMIUM Verified™ collaborator-tested data |
| Catalogue reference | M00135 |
| Product category | Primary Antibodies, Rabbit Monoclonal Antibodies |
| Reactive species | Human, Mouse, Rat |
| Tested applications | Flow Cytometry, IHC, IP, WB |
| Available conjugations | Unconjugated |
| Gene name | EIF4E |
| Protein name | Eukaryotic translation initiation factor 4E |
| Immunogen | A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Purification | Affinity-chromatography |
| Storage | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| UniProt | P06730 |
| NCBI Gene ID | 1977 |
| Conjugation | Size |
|---|---|
| Unconjugated | 100 μl/vial |
| Collaborator | Boster Bio |
| Partner model | M00135 |
| Target | Eukaryotic translation initiation factor 4E |
| Reactivity | Human,Mouse,Rat |
| Applications | Flow Cytometry,IHC,IP,WB |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1000-5000<br>IHC 1:50-200<br>IP 1:30<br>FC 1:40<br> |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | Clone: AFHI-5 |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. |
| Concentration | 0.5mg/ml |
| Purification | Affinity-chromatography |
| Shelf life | Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles. |
| Availability | Available |
| Species | WB | IHC | IHC-P | IHC-F | IF/ICC | Flow Cytometry | ELISA | IP |
|---|---|---|---|---|---|---|---|---|
| Human | ||||||||
| Mouse | ||||||||
| Rat |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
For research applications. Not for diagnostic use.
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