Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-29659

Anti-E2F4 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-E2F4 Monoclonal Antibody Boster Bio
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Anti-E2F4 Monoclonal Antibody Boster Bio

Product Description

Anti-E2F4 Monoclonal Antibody is a research-use antibody from Boster Bio for studying Transcription factor E2F4 in Flow Cytometry, IF/ICC, IHC, WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-E2F4 Monoclonal Antibody catalog # M01920. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M01920
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human
Tested applications Flow Cytometry, IF/ICC, IHC, WB
Available conjugations Unconjugated
Gene name E2F4
Protein name Transcription factor E2F4
Immunogen A synthesized peptide derived from human E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt Q16254
NCBI Gene ID 1874

Available variants

Conjugation Size
Unconjugated 100 μl/vial

Key Facts

Product typePrimary Antibody Target: Transcription factor E2F4
Collaborator Boster Bio
Partner model M01920
Target Transcription factor E2F4
Reactivity Human
Applications Flow Cytometry,IF/ICC,IHC,WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000<br>IHC 1:50-200<br>ICC/IF 1:50-200<br>FC 1:50<br>
Host Rabbit
Clonality Monoclonal
Clone Clone: AFDF-5
Isotype Rabbit IgG
Immunogen A synthesized peptide derived from human E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
Concentration 0.5mg/ml
Purification Affinity-chromatography
Shelf life Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

1 species
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human

Specifications

Target
Transcription factor E2F4
Also known as
E2F 4; E2F4; Transcription factor E2F4
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: AFDF-5
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human
Tested applications
Flow CytometryIF/ICCIHCWB
Immunogen
A synthesized peptide derived from human E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.
Observed band
85 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Purification
Affinity-chromatography

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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