Collaborator Boster Bio
Primary AntibodyABMIUM Verified™Collaborator Tested
ABM-29473

Anti-BOB1 Monoclonal Antibody

ABMIUM Verified™|Collaborator Tested|Boster Bio

Primary Antibody supplied by Boster Bio Read more ›

Anti-BOB1 Monoclonal Antibody Boster Bio
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Anti-BOB1 Monoclonal Antibody Boster Bio

Product Description

Anti-BOB1 Monoclonal Antibody is a research-use antibody from Boster Bio for studying POU domain class 2-associating factor 1 in IHC, IP, WB workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Boster Bio Anti-BOB1 Monoclonal Antibody catalog # M04431. Tested in WB, IHC, IP applications. This antibody reacts with Human, Rat.

Product information

Supplier Boster Bio
ABMIUM evidence status ABMIUM Verified™ collaborator-tested data
Catalogue reference M04431
Product category Primary Antibodies, Rabbit Monoclonal Antibodies
Reactive species Human, Rat
Tested applications IHC, IP, WB
Available conjugations Unconjugated
Gene name POU2AF1
Protein name POU domain class 2-associating factor 1
Immunogen A synthesized peptide derived from human BOB1 Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Host Rabbit
Clonality Monoclonal
Isotype Rabbit IgG
Purification Affinity-chromatography
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
UniProt Q16633
NCBI Gene ID 5450

Available variants

Conjugation Size
Unconjugated 100 μl/vial

Key Facts

Product typePrimary Antibody Target: POU domain class 2-associating factor 1
Collaborator Boster Bio
Partner model M04431
Target POU domain class 2-associating factor 1
Reactivity Human,Rat
Applications IHC,IP,WB
ConjugationUnconjugated
Dilution rangeWB 1:500-2000<br>IHC 1:50-200<br>IP 1:30<br>
Host Rabbit
Clonality Monoclonal
Clone Clone: ADEH-16
Isotype Rabbit IgG
Immunogen A synthesized peptide derived from human BOB1 Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Concentration 0.5mg/ml
Purification Affinity-chromatography
Shelf life Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
AvailabilityAvailable
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIHC-PIHC-FIF/ICCFlow CytometryELISAIP
Human
Rat

Specifications

Target
POU domain class 2-associating factor 1
Also known as
POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1; POU2AF1; OBF1
Concentration
0.5mg/ml
Form / buffer
Liquid
Host species
Rabbit
Clonality
Monoclonal
Clone number
Clone: ADEH-16
Isotype
Rabbit IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
IHCIPWB
Immunogen
A synthesized peptide derived from human BOB1 Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Observed band
37 kDa
Dilution range
WB 1:500-2000
IHC 1:50-200
IP 1:30
Purification
Affinity-chromatography

Storage & Stability

Shipped at
Ambient or cold-chain dependent on product format
Short-term storage
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Storage buffer
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Shelf life
Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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