Home » 53BP1 (phospho-Ser1778) rabbit pAb
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Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96854

53BP1 (phospho-Ser1778) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

53BP1 (phospho-Ser1778) rabbit pAb from ELK Biotechnology Read more ›

53BP1 (phospho-Ser1778) rabbit pAb
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53BP1 (phospho-Ser1778) rabbit pAb

Product Description

53BP1 (phospho-Ser1778) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 53BP1 in WB workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human 53BP1 (phospho-Ser1778)
Validation evidence captions Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody› Target: 53BP1
Collaborator ELK Biotechnology
Partner model ES18530
Target 53BP1
Research areas>>NOD-like receptor signaling pathway
Reactivity Human,Rat,Mouse
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human 53BP1 (Ser1778)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Cerebellum,Cervix,Epithelium,Myeloid leukemia cell,Skeletal muscle,
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000 —
Rat — —
Mouse — —

Specifications

Target
53BP1
Also known as
Tumor suppressor p53-binding protein 1 (53BP1) (p53-binding protein 1) (p53BP1)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WB
Immunogen
Synthesized phosho peptide around human 53BP1 (Ser1778)
Observed band
213kD
Cellular localization
Nucleus . Chromosome . Chromosome, centromere, kinetochore . Localizes to the nucleus in absence of DNA damage (PubMed:28241136). Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs): recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600). Associated with kinetochores during mitosis (By similarity). .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified™

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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