Human KLF5(Kruppel Like Factor 5, Intestinal) Microsample ELISA Kit

Human KLF5(Kruppel Like Factor 5, Intestinal) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring KLF5 in Tissue homogenates, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLF5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include KLF5; BTEB2; CKLF; IKLF; Intestinal Enriched Krüppel-Like Factor; Colon krueppel-like factor; Basic Transcription Element Binding Protein 2; GC-box-binding protein 2.