Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM56318

Human TF(Thymic Factor) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Human TF(Thymic Factor) ELISA Kit from ELK Biotechnology Read more ›

Human TF(Thymic Factor) ELISA Kit
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Human TF(Thymic Factor) ELISA Kit

Product Description

Human TF(Thymic Factor) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring TF in Plasma samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TF in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III.

Key Facts

Product typeELISA Kits Target: TF
Collaborator ELK Biotechnology
Partner model ELK1247
Target TF
Research areasHematology, Cardiovascular biology
Reactivity Human
Applications ELISA
Assay type Sandwich
Sample types Plasma
Detection range 15.63-1000 pg/mL
Sensitivity 5.7 pg/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
TF
Also known as
CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
5.7 pg/mL
Detection range
15.63-1000 pg/mL
Sample types
Plasma
Plate format
96-well pre-coated
Species reactivity
Human

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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