Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM55730

Chicken VTG(Vitellogenin) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Chicken VTG(Vitellogenin) ELISA Kit from ELK Biotechnology Read more ›

Chicken VTG(Vitellogenin) ELISA Kit
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Chicken VTG(Vitellogenin) ELISA Kit

Product Description

Chicken VTG(Vitellogenin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring VTG in Serum, Plasma, Cell lysates, Cell culture supernatant, Other biological fluids samples from Chicken. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken VTG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken VTG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken VTG in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include vtg1; Vitellogenin; VTG;.

Key Facts

Product typeELISA Kits Target: VTG
Collaborator ELK Biotechnology
Partner model ELK11356
Target VTG
Research areasCancer, leukopenia
Reactivity Chicken
Applications ELISA
Assay type Competitive Inhibition
Sample types Serum,Plasma,Cell lysates,Cell culture supernatant,Other biological fluids
Detection range 0.32-20 ng/mL
Sensitivity 0.115 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
VTG
Also known as
vtg1; Vitellogenin; VTG;
Form / buffer
Pre-coated microplate kit
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.115 ng/mL
Detection range
0.32-20 ng/mL
Sample types
SerumPlasmaCell lysatesCell culture supernatantOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Chicken

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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