Rat a2M(Alpha-2-Macroglobulin) ELISA Kit from ELK Biotechnology Read more ›
Rat a2M(Alpha-2-Macroglobulin) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring a2M in Serum, Plasma, Urine, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat a2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat a2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat a2M in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5.
| Collaborator | ELK Biotechnology |
| Partner model | ELK10817 |
| Target | a2M |
| Research areas | Tumor immunity, Infection immunity, Hematology |
| Reactivity | Rat |
| Applications | ELISA |
| Assay type | Sandwich |
| Sample types | Serum,Plasma,Urine,Other biological fluids |
| Detection range | 7.82-500 µg/mL |
| Sensitivity | 2.2 µg/mL |
| Sample Type | SERUM | PLASMA (EDTA) | URINE | CSF | CELL SUPERNATANT |
|---|---|---|---|---|---|
| Sample compatibility |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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