Collaborator ELK Biotechnology
ELISA KitsISO 9001|ABMIUM Verified™
ABM52488

Mouse CTSL(Cathepsin L) ELISA Kit

ISO 9001|ABMIUM Verified™|ELISA

Mouse CTSL(Cathepsin L) ELISA Kit from ELK Biotechnology Read more ›

Mouse CTSL(Cathepsin L) ELISA Kit
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Mouse CTSL(Cathepsin L) ELISA Kit

Product Description

Mouse CTSL(Cathepsin L) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring CTSL in Serum, Plasma, Tissue homogenates, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CTSL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CTSL in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include CTSL1; CATL; CTS-L; MEP; Cathepsin L1; Major excreted protein.

Key Facts

Product typeELISA Kits Target: CTSL
Collaborator ELK Biotechnology
Partner model ELK10487
Target CTSL
Research areasTumor immunity, Infection immunity
Reactivity Mouse
Applications ELISA
Assay type Sandwich
Sample types Serum,Plasma,Tissue homogenates,Other biological fluids
Detection range 0.16-10 ng/mL
Sensitivity 0.062 ng/mL
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Sample Type Validation

1 sample type
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
Sample TypeSERUMPLASMA (EDTA)URINECSFCELL SUPERNATANT
Sample compatibility

Specifications

Target
CTSL
Also known as
CTSL1; CATL; CTS-L; MEP; Cathepsin L1; Major excreted protein
Form / buffer
Pre-coated microplate kit
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.062 ng/mL
Detection range
0.16-10 ng/mL
Sample types
SerumPlasmaTissue homogenatesOther biological fluids
Plate format
96-well pre-coated
Species reactivity
Mouse

Storage & Stability

Long-term storage
Store according to the conditions stated in the product manual.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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