Recombinant Hirudin protein

BT LAB recombinant protein packaging — manufacturer reference image

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Recombinant Hirudin protein

Recombinant Hirudin protein

SKU:
ABM223517
Manufacturer SKU
CD02411
Product type
Recombinant Proteins
TargetRecombinant Hirudin protein
Expression systemPichia Pastoris
Purity> 96 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Hirudin is the most potent natural thrombin-specific protease inhibitor, and is originally derived in the salivary glands of the medicinal leech. Unlike heparin, hirudin act directly on thrombin, rather than through other clotting factors. They have a high binding affinity and specificity for thrombin. Therefore, hirudin prevents or dissolves the formation of clots and thrombi, and has therapeutic value in blood coagulation disorders, in the treatment of skin hematomas and of superficial varicose veins, either as an injectable or a topical application cream.

Expression system
Pichia Pastoris
Purity
> 96 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.
Endotoxin
Less than 1 EU/μg of rHirudin as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Cardiovascular
Also known as
Hirudin, Recombinant
Molecular weight
Approximately 6.7 kDa, a single non-glycosylated polypeptide chain containing 63 amino acid residues.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.
Activity
The biological activity is determined by chromogenic assay, 1 unit is defined as the amount of Hirudin that neutralizes 1 unit of the WHO preparation 89/588 of thrombin. The specific activity is no less than 14,000 ATU/mg protein.
Endotoxin level
Less than 1 EU/μg of rHirudin as determined by LAL method.
Sequence
VVYTDCTESG QNLCLCEGSN VCGQGNKCIL GSDGEKNQCV TGEGTPGPQS HNDGDFEEPE EYL
Quantity per pack
10 ug, 2 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD02411
UniProt accession
P01050
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM PBS, pH 7.0, containing 2 % mannitol.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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