Recombinant Human FGF-4 protein

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Recombinant Human FGF-4 protein

Recombinant Human FGF-4 protein

SKU:
ABM222338
Manufacturer SKU
CD01801
Product type
Recombinant Proteins
TargetRecombinant Human FGF-4 protein
Expression systemEscherichia coli.
Purity> 96 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

FGF4, also named FGF-K and K-FGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF-4 (71-206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with Mouse, rat, canine and bovine FGF-4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.

Expression system
Escherichia coli.
Purity
> 96 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Endotoxin
Less than 1 EU/μg of rHuFGF-4 as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Immunology
Also known as
FGF-4, FGF4, HBGF-4, HST, HST-1, HSTF-1, HSTF1, K-FGF, KFGFHuman
Source organism
Human
Molecular weight
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.
Activity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Endotoxin level
Less than 1 EU/μg of rHuFGF-4 as determined by LAL method.
Sequence
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Quantity per pack
5 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD01801
UniProt accession
P08620
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

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