Recombinant Human HMGB1 protein (C-6His)

Background

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins. Human HMGB1 is expressed as a 30 kDa, 215 amino acid (a.a.) single chain polypeptide containing three domains: two N-terminal globular, 70 a.a. positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 a.a. C-terminal region that contains only Asp and Glu.4, 5 Residues 27 - 43 and 178 - 184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues. HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins.

Expression system

Escherichia coli.

Purity

> 95 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 26.0 kDa, a single non-glycosylated polypeptide chain containing 223 amino acids with 6 × His at C-terminus.

Endotoxin

Less than 1 EU/μg of rHuHMGB1, His as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

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