Recombinant Human Midkine protein

Background

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % identity between the human and Mouse proteins.

Expression system

Escherichia coli.

Purity

> 97 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 13.4 kDa, a single non-glycosylated polypeptide chain containing 123 amino acids.

Endotoxin

Less than 1 EU/μg of rHuMidkine as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4.

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