Recombinant Human MIP-1 Beta/CCL4 protein

Background

CCL4 encoded by the CCL4 gene, also known as Macrophage Inflammatory Protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. It is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Specifically, MIP-1-beta (3-69) is also a ligand for CCR1 and CCR2 isoform B. Recombinant human CCL4 contains 69 amino acids and it shares 77 % and 80 % a.a. sequence identity with Mouse and rat CCL4, respectively. Both human and Mouse MIP-1α and MIP-1β are active on human and Mouse hematopoietic cells.

Expression system

Escherichia coli.

Purity

> 96 % by SDS-PAGE and HPLC analyses.

Molecular weight

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

Endotoxin

Less than 1 EU/ug of rHuMIP-1β/CCL4 as determined by LAL method.

Form

Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

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