Recombinant Mouse MIP-3 Beta/CCL19 protein

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Recombinant Mouse MIP-3 Beta/CCL19 protein

Recombinant Mouse MIP-3 Beta/CCL19 protein

SKU:
ABM219459
Manufacturer SKU
CD00293
Product type
Recombinant Proteins
TargetRecombinant Mouse MIP-3 Beta/CCL19 protein
Expression systemEscherichia coli.
Purity> 97 % by SDS-PAGE and HPLC analyses.

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

CCL19, also known as EBI1 Ligands chemokine (ELC) and macrophage inflammatory protein-3-beta, is encoded by the CCL19 gene which is located on chromosome 4 in mouse. The protein is expressed at high levels in thymus and lymph nodes, with intermediate levels in trachea and colon and low levels in stomach, lung and some other organs. CCL19 is signal through CCR7 and chemotaxis of T and B lymphocytes and myeloid progenitor cells.

Expression system
Escherichia coli.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Molecular weight
Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.
Endotoxin
Less than 1 EU/μg of rMuMIP-3β/CCL19 as determined by LAL method.
Form
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Packaging image shown for reference. Select a size from the available options.

Key facts & specifications
Research areas
Immunology
Also known as
MIP-3β/CCL19, CKb11, ELC, MIP-3b, MIP3B, SCYA19,Mouse
Source organism
Mouse
Molecular weight
Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.
Form / buffer
Sterile Filtered White lyophilized (freeze-dried) powder. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml.
Endotoxin level
Less than 1 EU/μg of rMuMIP-3β/CCL19 as determined by LAL method.
Sequence
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS
Quantity per pack
5 ug
Technical note
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 C. Further dilutions should be made in appropriate buffered solutions.
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSDS-PAGEHPLCLALBioactivity
Quality control
Identifiers & database references
Manufacturer SKU
CD00293
Storage, stability & shipping
Storage conditions
-20ºC
Storage buffer
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
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