Human Interleukin 21 Receptor, IL21R ELISA Kit

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Human Interleukin 21 Receptor, IL21R ELISA Kit

Human Interleukin 21 Receptor, IL21R ELISA Kit

SKU:
ABM218396
Manufacturer SKU
E6614Hu
Product type
ELISA Kits
TargetIL21R
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Cell culture supernatants, Ascites, Tissue homogenates

Manufacturer provenance

Manufactured by:
BT LAB
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Background

Interleukin 21 receptor is a type I cytokine receptor. IL21R is its human gene. The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2 (IL2), interleukin 7 (IL7) and interleukin 15 (IL15). This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells.

Test principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human IL21R antibody. IL21R present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IL21R Antibody is added and binds to IL21R in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL21R antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human IL21R. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Key facts & specifications
Research areas
Immunology
Also known as
CD antigen CD360;CD360;IL-21 receptor;IL21R;IL-21R;IMD56;Interleukin-21 receptor;NILR;Novel interleukin receptor;
Assay type
Sandwich
Detection method
Colorimetric (450 nm)
Sensitivity
11.37ng/L
Detection range
20-3500ng/L
Plate format
Pre-coated ELISA plate
Assay duration
1h 30m
Molecular weight
59,130 Da
Quantity per pack
48T, 96T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

EvidenceSerumPlasmaCell culture supernatantsAscitesTissue homogenates
Compatibility
Identifiers & database references
Manufacturer SKU
E6614Hu
Gene symbol
IL21R
Gene ID
50615
UniProt accession
Q9HBE5
Storage, stability & shipping
Shelf life
Store at -20°C for one year. Or store at 2-8°C for 6 months. If individual reagents are opened it is recommended that the kit be used within 1 month. Avoid repeated thaw cycles. *This product is for research use only, not for use in diagnosis procedures. It’s highly recommended to read this instruction entirely before use.
Storage conditions
2-8ºC
Compliance & documents
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by BT LAB and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

BT LAB
£240.93£205.00